Heterocyclic Schiff base derivatives containing pyrazolone moiety: Synthesis, characterization, and in vitro biological studies

In this study, some pyrazolone-based Schiff base derivatives 2a-e (except 2a) were synthesized for the first time and structurally illuminated by spectroscopic techniques (1H, 13C NMR, FT-IR) elemental analysis. All molecules screened their anticancer antioxidant activities, as well AChE, BChE, tyrosinase inhibitory properties. The obtained results exhibited that compounds 1b (IC50 = 9.497 μΜ) 2a 30.49 significantly decreased proliferation of HeLa cells. On other hand, apoptotic effects these two investigated with acridine orange/propidium iodide double staining. cell ratios treated determined 60 64%. Compound 2b 17.95 ± 0.47 was to be a very active substance in ABTS assay. 2e (A0.5 43.75 0.62 μM) indicated closest activity standard compound α-TOC 50.58 0.39 cupric reducing capacity (CUPRAC) inhibition studies enzymes, it 2c had molecule activities 82.79 1.03, 91.39 1.06, 92.60 1.80% inhibition, respectively. It target showed better enzyme than those ester 1a-e.